A molecular analysis of Escherichia coli O157:H7 strains isolated from cow and buffalo milk by the use of PCR in Karbala province

This study was conducted at Karbala governorate through the months of November 2021 until March 2022. Total number 240 sampling carried out from street vender and local store (milk, yogurts cheese) cow buffalo. These samples were collected different locations in Iraqi province. Escherichia coli O157H7 serotype prevalence rate buffalo major focus this research conducted. 120 products with regions (local shops vendors) consisting a total samples. After initial enrichment, streaked on Chromagar TM E. O157:H7 sorbitole Maconkey agar. O157h7 isolates serotyped for to be detectable o157 h7 antigens non-sorbitol fermenting (NSF) Isolates. Additionally, virulent factor examined. (rfbO157 fliCH7) genes by PCR procedures. 14 11% 18 (15%) All fourteen produced milk became observed positive (rfbO157andfliCH7) (100%) genes, but just 13